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Flashcards covering cell cloning, gene cloning, organismal cloning, nuclear transplantation, stem cells, cell cycle, oncogenes, and tumor suppressor genes.
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Cell cloning
Division of asexually reproducing cell - Binary fission in bacteria - Mitosis or budding in yeast - Produces two genetically identical daughter cells
Gene cloning
DNA cloning that produces multiple copies of a single gene using a cloning vector
Organismal Cloning
Production of organism (s) that are genetically identical to an existing organism
Differentiated cell
A cell that has undergone changes in gene expression to become a more specific type of cell
Nuclear transplantation
Method where nucleus of a donor cell is relocated to a target cell that has had its nucleus removed; technique used to create the first cloned mammal (sheep)
Embryonic stem cells
Capable of differentiation into many types of specialized cells (pluripotent); can generate all cell types
Adult stem cells
Can differentiate into a limited number of specialised cell types
Totipotent cells
Can generate a complete new organism; can give rise to ALL parts of the embryo and adult
Pluripotent cells
Can differentiate into many, but not all parts of an organism
Induced pluripotent stem (iPS) cells
Differentiated cells that are reprogrammed into cells the act like embryonic stem
Therapeutic cloning
Embryonic stem cells use to treat disease
Reproduction cloning
using of cloning techniques to make new individuals
Cell cycle checkpoints
Prevents cells from dividing when they lack energy or materials
Oncogene
Cancer causing gene; that arise from mutations in proto-oncogenes which lead to an increase in activity or amount of proteins regulating cell division
Proto-oncogene
Normal version of a gene that encodes for a protein that regulated cell growth and division
Tumor-Suppressor genes
Genes that encode for proteins that prevent uncontrolled cell growth through repairing damaged DNA, controlling cell adhesion, and inhibiting the cell cycle
Ras Gene
Stimulates the cell cycle; mutation: becomes an oncogene, the G-protein product will be more active and there will be increased cell division
P53 gene
Tumor-suppressor gene; inhibits the cell cycle, mutation: protein product will be less active and there will be an increase in cell division