Cell Cloning, Gene Cloning, and Cancer Development

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Flashcards covering cell cloning, gene cloning, organismal cloning, nuclear transplantation, stem cells, cell cycle, oncogenes, and tumor suppressor genes.

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18 Terms

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Cell cloning

Division of asexually reproducing cell - Binary fission in bacteria - Mitosis or budding in yeast - Produces two genetically identical daughter cells

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Gene cloning

DNA cloning that produces multiple copies of a single gene using a cloning vector

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Organismal Cloning

Production of organism (s) that are genetically identical to an existing organism

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Differentiated cell

A cell that has undergone changes in gene expression to become a more specific type of cell

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Nuclear transplantation

Method where nucleus of a donor cell is relocated to a target cell that has had its nucleus removed; technique used to create the first cloned mammal (sheep)

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Embryonic stem cells

Capable of differentiation into many types of specialized cells (pluripotent); can generate all cell types

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Adult stem cells

Can differentiate into a limited number of specialised cell types

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Totipotent cells

Can generate a complete new organism; can give rise to ALL parts of the embryo and adult

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Pluripotent cells

Can differentiate into many, but not all parts of an organism

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Induced pluripotent stem (iPS) cells

Differentiated cells that are reprogrammed into cells the act like embryonic stem

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Therapeutic cloning

Embryonic stem cells use to treat disease

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Reproduction cloning

using of cloning techniques to make new individuals

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Cell cycle checkpoints

Prevents cells from dividing when they lack energy or materials

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Oncogene

Cancer causing gene; that arise from mutations in proto-oncogenes which lead to an increase in activity or amount of proteins regulating cell division

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Proto-oncogene

Normal version of a gene that encodes for a protein that regulated cell growth and division

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Tumor-Suppressor genes

Genes that encode for proteins that prevent uncontrolled cell growth through repairing damaged DNA, controlling cell adhesion, and inhibiting the cell cycle

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Ras Gene

Stimulates the cell cycle; mutation: becomes an oncogene, the G-protein product will be more active and there will be increased cell division

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P53 gene

Tumor-suppressor gene; inhibits the cell cycle, mutation: protein product will be less active and there will be an increase in cell division

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